Blotting Reagents & Membranes Stripping Reagent
Efficient Stripping Buffer
EzReprobe is a high performance stripping reagent for western blotting
Purpose and Application
|Major components||Buffer, Surfactant|
|Method of use||Working solution：Mix 100mL of EzReprobe with 0.6g of enhancer|
|Storage period||At room temperature for 1 year|
|Package||EzReprobe reagent: 500 mL
Enhancer (Powder): 3g
EzReprobe is suitable for the following situations.
1. Immense membrane after emission detection in EzReprobe solution and shake it to incubate for 15 min at room temperature.
*Incubation time depends on antibody titer.
*In the case of antibody having high antibody titer or difficult to come off, incubation time should be extended.
2. After cleaning with wash buffer, the step after blocking is performed for antigen-antibody reaction.
Even rabbit monoclonal antibody having high antibody titer can be stripped with EzReprobe.
Also, denaturation of antigen is minimized.
*Red arrow head: Shows the band of target antigen of each step.
*Grey arrow head: Shows the band result from antibody cannot be peeled off by stripping operation.
*Non-Treated: Shake it to incubate at room temperature with wash buffer (TBST)
*Handmade: Shake it to incubate for 30 min at 50 ℃ (refer to Kaufmann et al., 1987)