Pre-wet PVDF Membrane ＆ Filter paper for Semi-Dry blotting
Blotting Reagents & Membranes Transfer Buffer
WSE-4055QBlot Kit
Needless preparation！Use pre-wet PVDF membrane＆ filter paper immediately.
「QBlot Kit」is prepared pre-wet PVDF membrane & filter paper.「QBlot Kit」is composited 10 stacks/box.
1 stack for 1 transfer and 「QBlot Kit」may use all Semi-Dry blotting systems.
Shortest 5-10 min until 250kDa protein transfer with ATTO High-Speed transfer blotting system！
WSE-4055 QBlot Kit
10 stacks/Box
Purpose and Application
Semi-Dry blotting
ATTO Mini gel size（8.5×9cm）
Pre-wet PVDF membrane ＆ filter paper
Product Summary
・Ready to use
・Faster transfer : Molecular weight ～250kDa！
・Prevention for transfer non-uniformity / pattern falling out by bobble
・Save a time with convenient！
・Alcohol free
Features
Western blotting is needed a workflow for equilibrating membrane and filter paper. Normally, the equilibration is taken 15～30 min.
QBlot kit is needless the equilibration and it may use for western blotting immediately.
ATTO made shortest time for transfer 5-10 min with good transfer efficiency.
「QBlot Kit」not only ATTO Semi-dry blotting system but also,「QBlot Kit」may use other semi-dry transfer system as well.
Specifications
| Model name | WSE-4055 QBlot Kit |
| Composition |
Top Stack 90×85mm（10 pouch bags） Bottom Stack 90×85mm（10 pouch bags） Gel Wash Buffer（5×）100mL |
| Term | 1 year（Cold storage） |
| Volume | 10 stacks/box |
1 transfer: Use 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack
*Material detail
ATTO PVDF Membrane: WSE-4051 ClearBlot P+Membrane（pore size 0.2μm）
Filter paper is used non-woven(High quality absorbency) for QBlot Kit only
ATTO Gel Wash Buffer: 5x Dilution with DW
How to use
Take "Bottom Stack" out
Rolling it to removing a bobble
Layout it with sample gel
Rolling it to removing a bobble
Take "Top Stack" out
Overlay "Top Stack"
Rolling it to removing a bobble
Set "Top electrode cover"(ATTO blotting system) and start "Blotting"
Transfer condition
ATTO Mini gel (1pc)
| Standard（C.V） | 12V（300～800mA） 15～30 min |
| Standard（C.V） | 600mA（10～30V） 15～30 min |
| High Speed（C.V） | 24V（600～1600mA） 5～10 min |
Manual
Download
You can download various documents (PDF)
