Electrophoresis Reagents Sample Preparation
AE-1435 EzApply 2D Kit
Sample preparation buffer for 2D
EzApply 2D Kit is designed to extract proteins from mammalian tissues/cells in a serial manner and prepare samples for 2-D electrophoresis.
AE-1435 EzApply 2D Kit
Purpose and Application
Features
- Separate extraction allows higher protein concentration per gel, spot detection is easy.
- Reducing reagent, alkylating reagent, washing buffer and stratified solutions are also incorporated into a kit
- Low running cost, while allowing short-time extraction and easy sample preparation
- Combination use with AE-1430EzApply (sample preparation kit for SDS-PAGE) makes it applicable to SDS-PAGE as well as 2-D electrophoresis.
Specifications
| Product Number | AE-1435 | |
|---|---|---|
| Product Name | EzApply2DKit | |
| Major components | Wash buffer Solution 1 Solution 2 Alkylated reagent Stratified solutions |
Tris, NaCl Tris, Ampholine , DTT Urea, thiourea, surfactant, Ampholine , DTT Acrylamide, BPB Urea |
| Manner of package | Solution/30 mL bottle x 2 Solution/20 mL bottle Solution/10 mL bottle Solution/1 mL tube Solution/500 μL tube Powder/tube |
(Wash Buffer) (Solution 1) (Solution 2) (Alkylated reagent) (Stratified solution) (DTT) |
| Method of use | Appropriate amount of solution is applied to tissues or cells, which were lysed and extracted. | |
| Applicable amount | Extracted from 100 mg of mammalian tissue 20 runs. | |
| Storage period | Frozen at -20ºC for 6 months (before dissolved with DTT) or 1 week after dissolved with DDT | |
Documents
Instruction Manual
Download
You can download various documents (PDF)
